PUMA Antikörper (C-Term)

Produktnummer ABIN7464435

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PUMA in WB, IP und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PUMA Antikörper (C-Term) (ABIN7464435)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PUMA Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Human

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PUMA. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HCT116 cells with 30uM cisplatin treatment for 24hr , K562 , THP-1

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: BCL2 binding component 3

Hintergrund: BCL2 binding component 3 , JFY-1 , JFY1 , PUMA,This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Gen-ID: 27113

UniProt: Q96PG8, Q9BXH1

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity