beta Actin Antikörper (C-Term)

Produktnummer ABIN7464288

Dieses Maus Monoklonal-Antikörper erkennt spezifisch beta Actin in WB, IF, IHC (p), ICC und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für beta Actin Antikörper (C-Term) (ABIN7464288)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Güteklasse: KO Validated

Klon: GT5512

Produktdetails anti-beta Actin Antikörper

Bindungsspezifität: C-Term

Spezifität: IP/MS validation was supported by references (PMID:30377426)

Kreuzreaktivität: Cat, Hund, Drosophila melanogaster, Ziege, Human, Affe, Maus, Kaninchen, Ratte, Saccharomyces cerevisiae, Xenopus laevis, Zebrafisch (Danio rerio)

Aufreinigung: Affinity purified by Protein G.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 30 hpf zebrafish , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , rabbit blood , goat blood , MDA-MB-231

Validation: IP/MS, KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: actin beta

Hintergrund: Actin beta , BRWS1 , PS1TP5BP1,This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Molekulargewicht: 42 kDa

Gen-ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction