AFT1 Antikörper (pSer63)

Produktnummer ABIN7464253

Der Kaninchen Polyklonal Anti-AFT1-Antikörper wurde für WB validiert. Er ist geeignet, AFT1 in Proben von Human zu detektieren.

Kurzübersicht für AFT1 Antikörper (pSer63) (ABIN7464253)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AFT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-AFT1 Antikörper

Bindungsspezifität: pSer63

Spezifität: This antibody is raised against human ATF1 phosphorylated at Ser63, and it also may detect the phosphorylated CREB protein due to sequence homology.

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser63 of human ATF1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T (25 μg/ml anisomycin treatment for 30 min) , A431(200 ng/ml EGF treatment for 30 min) , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.94 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Andere Bezeichnung: activating transcription factor 1

Hintergrund: Activating transcription factor 1 , EWS-ATF1 , FUS/ATF-1 , TREB36,This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq]

Molekulargewicht: 29 kDa

Gen-ID: 466

UniProt: P18846

Pathways: Neurotrophin Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades