beta Actin Antikörper (C-Term)

Produktnummer ABIN7464220

Der Kaninchen Polyklonal Anti-beta Actin-Antikörper wurde für WB, IF, IHC (p), ICC, IP und IHC (fro) validiert. Er ist geeignet, beta Actin in Proben von Human zu detektieren.

Kurzübersicht für beta Actin Antikörper (C-Term) (ABIN7464220)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Güteklasse: KO Validated

Produktdetails anti-beta Actin Antikörper

Bindungsspezifität: C-Term

Spezifität: IP/MS validation was supported by references (PMID:30377395)

Kreuzreaktivität: C. elegans, Candida albicans, Cat, Huhn, Hund, Drosophila melanogaster, E. coli, Hamster, Pferd, Human, Affe, Maus, Onchocerca, Oryza sativa, Schwein, Pflanzen, Ratte, Saccharomyces cerevisiae, Schaf, Tree shrew, Xenopus laevis

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T , A431 , Jurkat , Raji , mouse kidney , mouse lung , mouse testis , Candida albicans (sc5314) , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , Rat-2 , NTH-3T3 , mouse brain , PC-12 , Rat brain

Validation: IP/MS, KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.4 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: actin beta

Hintergrund: Actin beta , BRWS1 , PS1TP5BP1,This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Molekulargewicht: 42 kDa

Gen-ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction