Nanog Antikörper

Produktnummer ABIN7464135

Dieser Maus Monoklonal Antikörper detektiert spezifisch Nanog in WB, IF, FACS und ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Nanog Antikörper (ABIN7464135)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Güteklasse: KO Validated

Klon: GT3312

Produktdetails anti-Nanog Antikörper

Kreuzreaktivität: Human, Maus

Aufreinigung: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: Nanog homeobox

Hintergrund: Nanog homeobox,Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Molekulargewicht: 35 kDa

Gen-ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance