Polybromo 1 Antikörper (AA 1639-1689)

Produktnummer ABIN7453903

Der Kaninchen Polyklonal Anti-Polybromo 1-Antikörper wurde für WB, IP und IHC (fp) validiert. Er ist geeignet, Polybromo 1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Polybromo 1 Antikörper (AA 1639-1689) (ABIN7453903)

Target: Polybromo 1 (PBRM1)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Polybromo 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-Polybromo 1 Antikörper

Bindungsspezifität: AA 1639-1689

Verwendungszweck: Rabbit anti-PBRM1 Antibody, Affinity Purified

Aufreinigung: Affinity Purified

Immunogen: Between AA 1639 and 1689

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:1000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu Polybromo 1

Target: Polybromo 1 (PBRM1)

Andere Bezeichnung: PBRM1

Hintergrund: Background: The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PBRM1 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PBRM1 has been proposed to function as a tumor suppressor protein. Recently, PBRM1 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21.

Gen-ID: 55193

UniProt: Q86U86