ARNT Antikörper (AA 1-50)

Produktnummer ABIN7453496

Der Kaninchen Polyklonal Anti-ARNT-Antikörper wurde für WB, IP und IHC (fp) validiert. Er ist geeignet, ARNT in Proben von Human zu detektieren.

Kurzübersicht für ARNT Antikörper (AA 1-50) (ABIN7453496)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARNT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-ARNT Antikörper

Bindungsspezifität: AA 1-50

Verwendungszweck: Rabbit anti-ARNT Antibody, Affinity Purified

Homologie: Bovine

Aufreinigung: Affinity Purified

Immunogen: between AA 1 and 50

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7453496 has not performed satisfactorily when used for WB of ARNT in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ARNT.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Andere Bezeichnung: ARNT

Hintergrund: Background: Aryl hydrocarbon receptor nuclear translocator (ARNT) is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. ARNT forms a complex with the ligand-bound Ah receptor, and is required for receptor function. ARNT has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1,12)(q21,p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia [taken from NCBI Entrez Gene (GeneID: 405)].

Gen-ID: 405

NCBI Accession: NP_001659

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt