Rekombinanter RPA2 Antikörper (pSer4, pSer8)

Produktnummer ABIN7453379

Der Kaninchen Monoklonal Anti-RPA2-Antikörper wurde für WB, IP, ICC und IF/ICC validiert. Er ist geeignet, RPA2 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter RPA2 Antikörper (pSer4, pSer8) (ABIN7453379)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser RPA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (fixed cells) (IF/ICC)

Klon: BL-165-5F1

Produktdetails anti-RPA2 Antikörper

Bindungsspezifität: pSer4, pSer8

Verwendungszweck: Rabbit anti-Phospho RPA32 (S4/S8) Recombinant Monoclonal Antibody [BL-165-5F1]

Immunogen: surrounding serine 4/8

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ICC-IF: 1:1,000 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IP: 6 μL/1.0 mg lysate

ICC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: RPA32

Hintergrund: Background: Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32 kDa (RPA2), and 14 kDa (RPA3). RPA2, the 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

Gen-ID: 6118

NCBI Accession: NP_002937

UniProt: P15927

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA