Chickens were immunized with HHHHHH conjugated with KLH (6xHIS) Eggs were collected and the Ig fraction prepared. Antibodies were solid phase adsorbed before immunoaffinity purified using the peptide immobilized on a solid phase matrix then conjugated with fluorescein isothiocyanate (FITC). Antibody concentration was determined using an absorbance at 280 nm: 1.32 equals 1.0 mg of IgY. FITC/Protein Ratio = 3.0 - 6.0.
This antibody will react with HHHHHH as determined by ELISA and IEP techniques. It is suitable for blotting, ELISA and IF applications. Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
PBS, pH 7.2
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.