ARNT Antikörper

Produktnummer ABIN7384450

Dieses Anti-ARNT-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von ARNT in WB und IHC (p). Geeignet für Human, Maus, Ratte und Hamster.

Kurzübersicht für ARNT Antikörper (ABIN7384450)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reaktivität: Human, Maus, Ratte, Hamster

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser ARNT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: R05-3B2

Produktdetails anti-ARNT Antikörper

Aufreinigung: Affinity Purified

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000 IHC 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Andere Bezeichnung: HIF1 beta

Hintergrund: ArnT,ARNT protein,ARNT,Aryl hydrocarbon receptor nuclear translocator,bHLHe2,Class E basic helix-loop-helix protein 2,Dioxin receptor,Dioxin receptor nuclear translocator,Drnt,HIF 1 beta,HIF 1beta,HIF-1-beta,HIF1-beta,HIF1B,HIF1beta,Hypoxia Inducible Factor 1,Hypoxia inducible factor 1 beta,Hypoxia-inducible factor 1-beta,nuclear translocator,Tango,Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex, ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8).

Molekulargewicht:

Observed_MW: 87kDa

Calculated_MW: 87kDa

Gen-ID: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt