Rekombinanter SLC17A7 Antikörper (Atto 488)

Produktnummer ABIN7272877

Der Atto 488-konjugierte Alpaca Monoklonal anti-SLC17A7 Antikörper (Klon Nb9) (ABIN7272877) detektiert spezifisch SLC17A7 in ICC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter SLC17A7 Antikörper (Atto 488) (ABIN7272877)

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Human

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser SLC17A7 Antikörper ist konjugiert mit Atto 488

Applikation: Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: Nb9

Produktdetails anti-SLC17A7 Antikörper (Atto 488)

Expressionssystem: E.coli

Spezifität: Human VGlut1, other species not yet tested, no significant cross-reaction with VGlut2 or VGlut9

Aufreinigung: Produced in: E.coli

Immunogen: anti-VGLUT1 is derived from a single-domain antibody (sdAb) recognizing specifically VGLUT1 with high affinity and specificity.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Details zu SLC17A7

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Andere Bezeichnung: VGlut1

Hintergrund: The vesicula glutamate transport 1 (vGlut1) is primarily located on the synaptic vesicles of glutamatergic synapses (excitatory). Its function is to fill the vesicles with the neurotransmitter glutamate before they fuse to the plasma membrane and release their content from the pre-synaptic compartment into the synaptic cleft to stimulate postsynaptic receptors. vGlut1 uses the energy from the proton gradient generated by the vesicular ATPase to transport glutamate inside the vesicle.