SLC17A7 Antikörper (AA 493-560) (HRP)

Produktnummer ABIN2483728

Produktdetails anti-SLC17A7 Antikörper (HRP)

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Bindungsspezifität: AA 493-560

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SLC17A7 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Detects ~52 kDa. No cross-reactivity against VGlut2.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Klon: S28-9

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2483728 was sufficient for detection of VGLut1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SLC17A7

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Andere Bezeichnung: VGLUT1 (SLC17A7 Produkte)

Synonyme: 2900052E22Rik antikoerper, AI851913 antikoerper, VGluT1 antikoerper, Vglut1 antikoerper, BNPI antikoerper, VGLUT1 antikoerper, slc17a7 antikoerper, vglut1 antikoerper, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antikoerper, solute carrier family 17 member 7 antikoerper, solute carrier family 17 (vesicular glutamate transporter), member 7a antikoerper, Slc17a7 antikoerper, SLC17A7 antikoerper, slc17a7a antikoerper

Hintergrund: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

Gen-ID: 116638

NCBI Accession: NP_446311

UniProt: Q62634