Rekombinanter SARS-CoV-2 Spike S1 Antikörper (RBD) (Atto 488)
Kurzübersicht für Rekombinanter SARS-CoV-2 Spike S1 Antikörper (RBD) (Atto 488) (ABIN7272865)
Target
Alle SARS-CoV-2 Spike S1 Antikörper anzeigenAntikörpertyp
Fragment
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- RBD
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Expressionssystem
- E.coli
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Spezifität
- RBD of S1 protein from SARS-CoV-2
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Aufreinigung
- Produced in: E.coli
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Immunogen
- anti-SARS-CoV-2 S1 is based on the W25 clone, which has a sub-nanomolar affinity for the SARS-CoV-2 S1 protein receptor binding domain (RBD) and can neutralize infections as it efficiently competes with ACE-2 receptor binding.
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Labeling Ratio
- a single fluorophore is coupled to exactly one sdAb
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Applikationshinweise
- IF: 1:1000 (corresponding to 5 nM final concentration)
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Kommentare
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Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.
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Buffer
- lyophilized from PBS pH7.4 with 2% BSA (US-Origin)
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Lagerung
- 4 °C/-20 °C/-80 °C
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Informationen zur Lagerung
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- SARS-CoV-2 Spike S1
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Andere Bezeichnung
- S1 protein from SARS-CoV-2
Target
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