TMC1 Antikörper (Extracellular)

Produktnummer ABIN7237916

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TMC1 in WB, FACS und LCI. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für TMC1 Antikörper (Extracellular) (ABIN7237916)

Target: TMC1 (Transmembrane Channel-Like 1 (TMC1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TMC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-TMC1 Antikörper

Bindungsspezifität: Extracellular

Verwendungszweck: A Rabbit Polyclonal antibody to TMC1 (extracellular) 

Spezifität: Extracellular, 3rd loop. 

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,human - identical

Produktmerkmale: Extracellular, 3rd loop

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EHDFPSWMAKILR, corresponding to amino acid residues 679 - 691 of mouse TMC1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Kommentare:

Negative Control: (ABIN7237509)

Blocking Peptide: (ABIN7237509)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu TMC1

Target: TMC1 (Transmembrane Channel-Like 1 (TMC1))

Andere Bezeichnung: TMC1 

Hintergrund: Transmembrane Channel Like 1, Transmembrane cochlear-expressed protein 1,Transmembrane channel-like protein 1 (TMC1) is one of eight mammalian TMC genes. Proteins that belong to this family contain six transmembrane domains with both the C and N termini on the cytoplasmic side of the membrane, as well as a large loop between domains 4 and 5. This multi-pass transmembrane topologic structure suggests roles as a receptor, transporter, channel, or pump1. TMC1 and the closely related TMC2 gene are assembled as dimers2 and are expressed in neurosensory hair cells of the mouse inner ear where they appear to have a role as pore forming units in mechanoelectrical transduction. Mutations of the transmembrane channel-like 1 (TMC1) gene can cause dominant and recessive forms of deafness in humans and mice3 and hence, identification of the molecular functions of Tmc genes is broadly significant because it may provide a handle on the fundamental mechanisms that mediate human hearing and balance4.

Alternative names: Transmembrane Channel Like 1, Transmembrane cochlear-expressed protein 1

Gen-ID: 13409

NCBI Accession: NM_138691

UniProt: Q8R4P5

Pathways: Sensory Perception of Sound