HOMER1 Antikörper (Intracellular)

Produktnummer ABIN7887048

Der Meerschweinchen Polyklonal anti-HOMER1 Antikörper wird verwendet zum Nachweis von HOMER1 in Proben von Ratte. Er wurde validiert für WB und IHC.

Kurzübersicht für HOMER1 Antikörper (Intracellular) (ABIN7887048)

Target: HOMER1 (Homer Homolog 1 (HOMER1))

Reaktivität: Ratte

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser HOMER1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-HOMER1 Antikörper

Bindungsspezifität: AA 293-296, Intracellular

Verwendungszweck: A Guinea Pig Polyclonal Antibody to Homer1

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): The antibody will not recognize Homer1 short isoform 3 (also known as Homer1a), but will recognize isoform 2 (also known as Homer1b).

Homologie: Mouse,human - identical

Produktmerkmale: Intracellular

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KQEIDNARELQEQR, corresponding to amino acid residues 293-296 of rat Homer1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235762)

Blocking Peptide: (ABIN7235762)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu HOMER1

Target: HOMER1 (Homer Homolog 1 (HOMER1))

Andere Bezeichnung: HOMER1

Hintergrund:

Synonyms: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47

Description: Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1.The long form of Homer1 assembles group 1 metabotropic glutamate receptors mGluR1 and mGluR5 in large macromolecular complexes at synapses as well as with components of the NMDA receptor complex at the neuronal cell membrane. In contrast, the short form of Homer1 lacks the ability of linking mGluR1/5 to synaptic proteins and functions as an endogenous negative modulator of the mGluR1/5-inositol 1,4,5-trisphosphate receptor signaling complex. Transgenic mice with overexpressed Homer1 in striatal medium spiny neurons demonstrate normal development of striatal structure and afferent/efferent connectivity. However, motor performance in behavioral tasks and striatal responses to the psychomotor stimulant amphetamine are significantly altered in these mice2.At concentrations

Gen-ID: 29546

NCBI Accession: NM_004272

UniProt: Q9Z214

Pathways: Skeletal Muscle Fiber Development