GJC2 Antikörper (Intracellular Loop)

Produktnummer ABIN7886198

Der Kaninchen Polyklonal anti-GJC2 Antikörper (ABIN7886198) detektiert spezifisch GJC2 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für GJC2 Antikörper (Intracellular Loop) (ABIN7886198)

Target: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GJC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GJC2 Antikörper

Bindungsspezifität: AA 100-111, Intracellular Loop

Verwendungszweck: A Rabbit Polyclonal antibody to Connexin-47 (GJC2)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Intracellular loop

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HRLARASEQERR, corresponding to amino acid residues 100 - 111 of rat Connexin-47

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235562)

Blocking Peptide: (ABIN7235562)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GJC2

Target: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Andere Bezeichnung: GJC2 

Hintergrund:

Synonyms: CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6

Description: Connexin-47 (Cx47) is a gap junction (GJ) protein expressed in the Central nervous system.It belongs to the connexins family of transmembrane proteins. Proteins that belong to this family form intercellular membrane channels that allow diffusional movement of ions, metabolites, and signaling molecules. The family members are composed of four transmembrane segments, an unstructured C and N cytoplasmic termini, a cytoplasmic loop and two extra-cellular loops1.Connexins oligomerize among the family members to form different structures and Cx47 was shown to form gap junction channels together with Cx43.These voltage gated channels exhibit ionic and chemical rectification, which creates a directional diffusion barrier for the movement of ions and negatively charged molecules from cells expressing Cx47 to those with Cx431.The channel mediates an intercellular communication between oligodendrocytes and between astrocytes. This process is essential to maintain the integrity of myelin and maintaining K+ buffering and nutrient homeostasis in oligodendrocytes, myelin and oligodendrocyte function2,3, and indeed, mutations in human Cx47 cause severe myelin disorders2.Cx43/Cx47 gap junction channels activity was shown to be associated with the development of Multiple Sclerosis and autoimmune demyelination, underscoring a critical role for Cx47 in regulating neuroinflammation4.

Gen-ID: 57165

NCBI Accession: NM_020435

UniProt: Q80XF7