GPR84 Antikörper (Extracellular) (PE)

Produktnummer ABIN7237670

Dieses Anti-GPR84-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GPR84 in FACS und LCI. Geeignet für Human.

Kurzübersicht für GPR84 Antikörper (Extracellular) (PE) (ABIN7237670)

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR84 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR84 Antikörper (PE)

Bindungsspezifität: AA 166-180, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR84 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human - 14,15 amino acid residues identical, mouse,rat - 13

Produktmerkmale: Extracellular, 2nd loop

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582046)

Blocking Peptide: (ABIN7235454)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR84

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Andere Bezeichnung: GPR84

Hintergrund: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33,GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.

Alternative names: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Gen-ID: 53831

NCBI Accession: NM_020370

UniProt: Q9NQS5