GPR84 Antikörper (2nd Extracellular Loop)

Produktnummer ABIN7886438

Der Kaninchen Polyklonal anti-GPR84 Antikörper (ABIN7886438) detektiert spezifisch GPR84 in WB, FACS, LCI, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für GPR84 Antikörper (2nd Extracellular Loop) (ABIN7886438)

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR84 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-GPR84 Antikörper

Bindungsspezifität: 2nd Extracellular Loop, AA 166-180

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR84

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human - 14,15 amino acid residues identical, mouse,rat - 13

Produktmerkmale: Anti-GPR84 (extracellular) Antibody (ABIN7043175, ABIN7044443 and ABIN7044444) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IP

Negative Control: (ABIN7235454)

Blocking Peptide: (ABIN7235454)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR84

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Andere Bezeichnung: GPR84

Hintergrund:

Synonyms: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Description: GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.

Gen-ID: 53831

NCBI Accession: NM_020370

UniProt: Q9NQS5