GABARAP Antikörper (C-Term)

Produktnummer ABIN7233059

Der Kaninchen Polyklonal Anti-GABARAP-Antikörper wurde für WB und IF/ICC validiert. Er ist geeignet, GABARAP in Proben von Human zu detektieren.

Kurzübersicht für GABARAP Antikörper (C-Term) (ABIN7233059)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABARAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Produktdetails anti-GABARAP Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-GABARAP Rabbit Polyclonal Antibody

Spezifität: This antibody recognizes human and mouse GABARAP.

Kreuzreaktivität: Human, Maus

Immunogen: Synthetic peptide corresponding to the C-terminus of human GABARAP

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at dilution of 1:1,000. A band of ~14-16 kDa is detected. Other bands observed at ~40 kDa (complex) and ~26 kDa (dimer).
Immunofluorescence: use at dilution of 1:100
These are recommended working dilutions.
Endusers should determine optimal dilutions for their applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -20°C.

Details zu GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Andere Bezeichnung: GABARAP

Hintergrund: γ-aminobutyric acid receptor-associated protein,Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355). Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538). Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205). Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205). Involved in apoptosis (PubMed:15977068). {PubMed:15169837, PubMed:15977068, PubMed:20562859, PubMed:22948227, PubMed:25684205, PubMed:31006538, PubMed:9892355}.,Gamma-aminobutyric acid A receptors (GABA(A) receptors) are ligand-gated chloride channels that mediate inhibitory neurotransmission. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. In addition, GABARAP has an important role in autophagy, it is crucial for autophagosome formation and sequestering cytosolic cargo into double-membrane vesicles which is then followed by degradation after fusion with lysosomes.,Cytoplasmic vesicle, autophagosome membrane, Endomembrane system, Cytoplasm, cytoskeleton, Golgi apparatus membrane, Cytoplasmic vesicle,GABA(A receptor-associated protein, MM46

UniProt: O95166

Pathways: Autophagie