RIPK2 Antikörper (pTyr474)

Produktnummer ABIN7232880

Dieses Anti-RIPK2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RIPK2 in WB und IHC. Geeignet für Human.

Kurzübersicht für RIPK2 Antikörper (pTyr474) (ABIN7232880)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RIPK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-RIPK2 Antikörper

Bindungsspezifität: pTyr474

Verwendungszweck: Anti-RIPK2 Rabbit Polyclonal Antibody

Spezifität: Human and mouse RIPK2.

Kreuzreaktivität: Human, Maus

Aufreinigung: Whole Antiserum

Immunogen: Phosphopeptide corresponding to the pY474 site of human RIPK2.

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at a dilution of 1:500. Detection of RIPK2 in MDP-stimulated HEK293 cells (+) vs. non-stimulated HEK293 cells (-)
These are recommended dilutions.
Endusers should determine optimal dilutions for their applications.
Immunohistochemistry: use at a dilution of 1:100. Detection of active RIPK2 in human (top) and mouse (bottom) tissues. Non-IBD refers to colon sections from non-inflammatory bowel disease (IBD) patients, IBD refers to colon sections from ulcerative colitis (UC) patients. Mouse strain used: B6.129P2-I110tm1Cgn/J, a common model for IBD that presents with spontaneous colitis that is elevated by DSS (dextran sulfate sodium) exposure.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: Whole antiserum

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C in appropriate aliquots to avoid multiple freeze-thaw cycles.

Details zu RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Andere Bezeichnung: RIPK2

Hintergrund: Receptor-interacting serine/threonine-protein kinase 2,Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed:26646181). {PubMed:14638696, PubMed:17054981, PubMed:18079694, PubMed:21123652, PubMed:21887730, PubMed:26646181}.,Cytoplasm

Gen-ID: 603455

UniProt: O43353

Pathways: T-Zell Rezeptor Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades