Immunoblotting: use at 1-10 μg/mL. A band of ~70 kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1 μg/mL Immunofluorescence: use at 1-10 μg/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: COS cell lysate transiently transfected with TrpM7.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Dilute in PBS or medium which is identical to that used in the assay system.
Konzentration
Lot specific
Buffer
PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
This antibody is stable for at least one (1) year at -20°C.
Heat sensitive channel TRPV3,Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. The TrpV3 protein belongs to a family of nonselective cation channels that function in a variety of processes including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin and are activated at distinct physiological temperatures. The TrpV3 channel is activated at temperatures between 22 and 40 C. The TrpV3 gene is in close proximity to the TrpV1 gene on chromosome 17, and studies suggest that the corresponding two proteins associate with each other to form heteromeric channels.