HYOU1 Antikörper

Produktnummer ABIN7232796

Der Maus Monoklonal anti-HYOU1 Antikörper (Klon 6E3-2C3) (ABIN7232796) detektiert spezifisch HYOU1 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für HYOU1 Antikörper (ABIN7232796)

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HYOU1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 6E3-2C3

Produktdetails anti-HYOU1 Antikörper

Verwendungszweck: Anti-Grp170 Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human, mouse, and rat Grp170 (170 kDa).

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein G affinity chromatography

Immunogen: Synthetic peptide sequence derived from human Grp170 (accession no. NP_006389.3)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA: confirmed with antibody (1-10 μg/mL) binding to Grp170 at 5 μg/mL on the solid phase
Immunoblotting: with antibody at 1.0-2.0 μg/mL. A band of 170 kDa is detected.
These are recommended concentrations
End user should determine optimal concentrations for their applications.
Positive control: HEK293 cell lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -20°C.

Details zu HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Andere Bezeichnung: HYOU1

Hintergrund: Hypoxia up-regulated protein 1,Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. {PubMed:10037731}.,Grp170 is a major chaperone/stress protein resident in the ER. It is distantly related in sequence to both hsp110 and hsp70 families, but represents the Grp170 stress protein family. Studies have suggested that Grp170 is an important element of the protein processing machinery of the ER. Unlike Hsp110 and Hsp70, Grp170 has two distinct chaperoning domains, a classic beta-sheet peptide binding domain, with some COOH- terminal sequence, and a COOH-terminal alpha-helical domain. Like other members of the Hsp70 superfamily, Grp170 can elicit anti-tumor immune responses most likely by chaperoning antigenic peptide and binding to receptor(s) on antigen-presenting cells (APC).,Endoplasmic reticulum lumen.,150 kDa oxygen-regulated protein, ORP-150, 170 kDa glucose-regulated protein, GRP-170

NCBI Accession: NP_006389

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interaktom