ZCCHC11 Antikörper

Produktnummer ABIN7231968

Der Kaninchen Polyklonal anti-ZCCHC11 Antikörper wird verwendet zum Nachweis von ZCCHC11 in Proben von Human und Maus. Er wurde validiert für WB und ELISA.

Kurzübersicht für ZCCHC11 Antikörper (ABIN7231968)

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZCCHC11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ZCCHC11 Antikörper

Verwendungszweck: Rabbit Anti-TUT4 Polyclonal Antibody

Spezifität: The antibody detects endogenous levels of TUT4 protein

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human TUT4 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu ZCCHC11

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Andere Bezeichnung: TUT4

Hintergrund: Terminal uridylyltransferase 4, TUTase 4, Zinc finger CCHC domain-containing protein 11ZCCHC11 is an RNA uridyltransferase (EC 2.7.7.52) that uses UTP to add uridines to the 3-prime end of substrate RNA molecules. GO annotations related to this gene include nucleic acid binding and nucleotidyltransferase activity. Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency. Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3 uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.

Molekulargewicht: 180kD

Gen-ID: 23318

UniProt: Q5TAX3

Pathways: Stem Cell Maintenance