WASF1 Antikörper (pTyr125)

Produktnummer ABIN7225724

Der Kaninchen Polyklonal anti-WASF1 Antikörper wird verwendet zum Nachweis von WASF1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für WASF1 Antikörper (pTyr125) (ABIN7225724)

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WASF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-WASF1 Antikörper

Bindungsspezifität: pTyr125

Verwendungszweck: Rabbit Anti-WAVE1 (phospho Tyr125) Polyclonal Antibody

Spezifität: Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human WAVE1 Phospho-Tyr125

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu WASF1

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Andere Bezeichnung: WAVE1

Hintergrund: WASF1, KIAA0269, SCAR1, WAVE1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Protein WAVE-1, Verprolin homology domain-containing protein 1The protein encoded by WASF1 (WAS protein family member 1), a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for WASF1.

Molekulargewicht: 70kD

Gen-ID: 8936

UniProt: Q92558

Pathways: RTK Signalweg, Regulation of Actin Filament Polymerization