UBE2O Antikörper

Produktnummer ABIN7232000

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch UBE2O in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für UBE2O Antikörper (ABIN7232000)

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE2O Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-UBE2O Antikörper

Verwendungszweck: UBE2O Polyclonal Antibody

Spezifität: The antibody detects endogenous levels of UBE2O protein

Aufreinigung: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human UBE2O protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu UBE2O

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Andere Bezeichnung: UBE2O

Hintergrund: Rabbit Anti-UBE2O Polyclonal Antibody,(E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, Ubiquitin-protein ligase O,E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27: mAGEL2 E3 ubiquitin ligase complex to mediate Lys-63-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.,UBE2O

Molekulargewicht: observerd band 142kDa

Gen-ID: 63893

UniProt: Q9C0C9