UBE3B Antikörper (AA 550-630)

Produktnummer ABIN7225756

Dieser Anti-UBE3B Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von UBE3B in WB, ELISA und IHC (p). Geeignet für Human.

Kurzübersicht für UBE3B Antikörper (AA 550-630) (ABIN7225756)

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-UBE3B Antikörper

Bindungsspezifität: AA 550-630

Verwendungszweck: UBE3B Polyclonal Antibody

Spezifität: UBE3B Polyclonal Antibody detects endogenous levels of UBE3B protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human UBE3B at AA range: 550-630

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu UBE3B

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Andere Bezeichnung: UBE3B

Hintergrund: Rabbit Anti-UBE3B Polyclonal Antibody,UBE3B, Ubiquitin-protein ligase E3B,The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: e1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. UBE3B (ubiquitin protein ligase E3B) encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for UBE3B.,Ubiquitin-protein ligase E3B

Molekulargewicht: observerd band 123kDa

Gen-ID: 89910

UniProt: Q7Z3V4