TOP2B Antikörper (AA 10-90)

Produktnummer ABIN7224754

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TOP2B in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für TOP2B Antikörper (AA 10-90) (ABIN7224754)

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-TOP2B Antikörper

Bindungsspezifität: AA 10-90

Verwendungszweck: Rabbit Anti-Topo IIβ Polyclonal Antibody

Spezifität: Topo IIβ Polyclonal Antibody detects endogenous levels of Topo IIβ protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Topo IIbeta at AA range: 10-90

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Andere Bezeichnung: Topo IIbeta

Hintergrund: TOP2B, DNA topoisomerase 2-beta, DNA topoisomerase II, beta isozymeTOP2B (topoisomerase (DNA) II beta) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. TOP2B encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in TOP2B have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of TOP2B results in two transcript variants, however, the second variant has not yet been fully described.

Molekulargewicht: 183kD

Gen-ID: 7155

UniProt: Q02880