TOP2B Antikörper (C-Term)

Produktnummer ABIN2855447

Produktdetails anti-TOP2B Antikörper

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Rabbit Polyclonal antibody to Topoisomerase II beta (topoisomerase (DNA) II beta 180 kDa)
Topoisomerase II beta antibody [C3], C-term

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Topoisomerase II beta. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Jurkat , K562 , THP-1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.48 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Andere Bezeichnung: DNA topoisomerase II beta (TOP2B Produkte)

Synonyme: TOP2B antikoerper, D230016L12Rik antikoerper, Top-2 antikoerper, TOPIIB antikoerper, top2beta antikoerper, DNA topoisomerase II beta antikoerper, topoisomerase (DNA) II beta antikoerper, microRNA 4442 antikoerper, TOP2B antikoerper, Top2b antikoerper, MIR4442 antikoerper

Hintergrund: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants, however, the second variant has not yet been fully described.

Cellular Localization: Cytoplasm , Nucleus , nucleolus

Molekulargewicht: 183 kDa

Gen-ID: 7155

UniProt: Q02880