SKP2 Antikörper (AA 340-420)

Produktnummer ABIN7217024

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SKP2 in WB, IHC, IF und ELISA. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für SKP2 Antikörper (AA 340-420) (ABIN7217024)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SKP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Produktdetails anti-SKP2 Antikörper

Bindungsspezifität: AA 340-420

Verwendungszweck: Rabbit Anti-Skp2 p45 Polyclonal Antibody

Spezifität: Skp2 p45 Polyclonal Antibody detects endogenous levels of Skp2 p45 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Skp2 p45 at AA range: 340-420

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Andere Bezeichnung: Skp2 p45

Hintergrund: SKP2, FBXL1, S-phase kinase-associated protein 2, Cyclin-A/CDK2-associated protein p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2SKP2 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. S-phase kinase-associated protein 2 encoded by this gene belongs to the Fbls class, in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms.

Molekulargewicht: 47kD

Gen-ID: 6502

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases