POLR2A/RPB1 Antikörper (pSer1619)

Produktnummer ABIN7223258

Dieser Anti-POLR2A/RPB1-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von POLR2A/RPB1 in WB, IF, ELISA und IHC (p). Geeignet für Human, Maus, Ratte und Affe.

Kurzübersicht für POLR2A/RPB1 Antikörper (pSer1619) (ABIN7223258)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-POLR2A/RPB1 Antikörper

Bindungsspezifität: pSer1619

Verwendungszweck: Rpb1 (phospho Ser1619) Polyclonal Antibody

Spezifität: Phospho-Rpb1 (S1619) Polyclonal Antibody detects endogenous levels of Rpb1 protein only when phosphorylated at S1619.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Rpb1 Phospho-Ser1619

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Andere Bezeichnung: Rpb1

Hintergrund: Rabbit Anti-Rpb1 (phospho Ser1619) Polyclonal Antibody,POLR2A, POLR2, DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1,POLR2A (RNA polymerase II subunit A) encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of POLR2A contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.,DNA-directed RNA polymerase II subunit RPB1

Molekulargewicht: observerd band 250kDa

Gen-ID: 5430

UniProt: P24928

Pathways: Regulatorische RNA Pathways