PRKAB1 Antikörper (pSer182)

Produktnummer ABIN7223438

Der Kaninchen Polyklonal Anti-PRKAB1 Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, PRKAB1 in Proben von Human, Maus, Ratte und Affe zu detektieren.

Kurzübersicht für PRKAB1 Antikörper (pSer182) (ABIN7223438)

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PRKAB1 Antikörper

Bindungsspezifität: pSer182

Verwendungszweck: AMPKβ1 (phospho Ser182) Polyclonal Antibody

Spezifität: Phospho-AMPKβ1 (S182) Polyclonal Antibody detects endogenous levels of AMPKβ1 protein only when phosphorylated at S182.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human AMPKbeta1 Phospho-Ser182

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu PRKAB1

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Andere Bezeichnung: AMPKbeta1

Hintergrund: Rabbit Anti-AMPKβ1 (phospho Ser182) Polyclonal Antibody,PRKAB1, AMPK, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb,The protein encoded by PRKAB1 (protein kinase AMP-activated non-catalytic subunit beta 1) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.,5'-AMP-activated protein kinase subunit beta-1

Gen-ID: 5564

UniProt: Q9Y478

Pathways: AMPK Signaling, Warburg Effekt