PRKAA1 Antikörper (pSer496)

Produktnummer ABIN7223434

Der Kaninchen Polyklonal Anti-PRKAA1 Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, PRKAA1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PRKAA1 Antikörper (pSer496) (ABIN7223434)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PRKAA1 Antikörper

Bindungsspezifität: pSer496

Verwendungszweck: AMPKα1 (phospho Ser496) Polyclonal Antibody

Spezifität: Phospho-AMPKα1 (S496) Polyclonal Antibody detects endogenous levels of AMPKα1 protein only when phosphorylated at S496.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human AMPKalpha1 Phospho-Ser496

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Andere Bezeichnung: AMPKalpha1

Hintergrund: Rabbit Anti-AMPKα1 (phospho Ser496) Polyclonal Antibody,PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1,The protein encoded by PRKAA1 (protein kinase AMP-activated catalytic subunit alpha 1) belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.,5'-AMP-activated protein kinase catalytic subunit alpha-1

Gen-ID: 5562

UniProt: Q13131

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt