PKA Antikörper (AA 1-80)

Produktnummer ABIN7216582

Der Kaninchen Polyklonal Anti-PKA Antikörper wurde für WB, ELISA, IHC (p) und IF validiert. Er ist geeignet, PKA in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PKA Antikörper (AA 1-80) (ABIN7216582)

Target: PKA (Protein Kinase A (PKA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-PKA Antikörper

Bindungsspezifität: AA 1-80

Verwendungszweck: PKAα/β cat Polyclonal Antibody

Spezifität: PKAα/β cat Polyclonal Antibody detects endogenous levels of PKAα/β cat protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human PKAalpha/beta cat at AA range: 1-80

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu PKA

Target: PKA (Protein Kinase A (PKA))

Andere Bezeichnung: PKAalpha/beta

Hintergrund: Rabbit Anti-PKAα/β cat Polyclonal Antibody,PRKACA, PKACA, cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alpha, PRKACB, cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta,PRKACA encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of PRKACA caused either by somatic mutations, or genomic duplications of regions that include PRKACA, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.,cAMP-dependent protein kinase catalytic subunit alpha

Molekulargewicht: observerd band 38kDa

Gen-ID: 5566, 5567

Pathways: SARS-CoV-2 Protein Interaktom