POLH Antikörper

Produktnummer ABIN7230724

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch POLH in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für POLH Antikörper (ABIN7230724)

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-POLH Antikörper

Verwendungszweck: Rabbit Anti-POLH Polyclonal Antibody

Spezifität: The antibody detects endogenous levels of POLH protein

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human POLH protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu POLH

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Andere Bezeichnung: POLH

Hintergrund: DNA polymerase eta, RAD30 homolog A, Xeroderma pigmentosum variant type proteinPOLH encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 78kD

Gen-ID: 5429

UniProt: Q9Y253

Pathways: DNA Reparatur