PPP1R12A Antikörper (pThr696)

Produktnummer ABIN7222634

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch PPP1R12A in ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PPP1R12A Antikörper (pThr696) (ABIN7222634)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPP1R12A Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PPP1R12A Antikörper

Bindungsspezifität: pThr696

Verwendungszweck: MYPT1 (phospho Thr696) Polyclonal Antibody

Spezifität: Phospho-MYPT1 (T696) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T696.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MYPT1 Phospho-Thr696

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Andere Bezeichnung: MYPT1

Hintergrund: Rabbit Anti-MYPT1 (phospho Thr696) Polyclonal Antibody,PPP1R12A, MBS, MYPT1, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit,Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP. hoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.,Protein phosphatase 1 regulatory subunit 12A

Gen-ID: 4659

UniProt: O14974

Pathways: M Phase