MCM3 Antikörper (Internal Region)

Produktnummer ABIN7217970

Der Kaninchen Polyklonal anti-MCM3 Antikörper (ABIN7217970) detektiert spezifisch MCM3 in WB, IHC, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für MCM3 Antikörper (Internal Region) (ABIN7217970)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-MCM3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Rabbit Anti-MCM3 Polyclonal Antibody

Spezifität: MCM3 Polyclonal Antibody detects endogenous levels of MCM3 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human MCM3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Andere Bezeichnung: MCM3

Hintergrund: MCM3, DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102Minichromosome maintenance complex component 3 encoded by MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 89kD

Gen-ID: 4172

UniProt: P25205

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA