LEF1 Antikörper (pSer42)

Produktnummer ABIN7222980

Der Kaninchen Polyklonal anti-LEF1 Antikörper wird verwendet zum Nachweis von LEF1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC.

Kurzübersicht für LEF1 Antikörper (pSer42) (ABIN7222980)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LEF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-LEF1 Antikörper

Bindungsspezifität: pSer42

Verwendungszweck: Rabbit Anti-LEF-1 (phospho Ser42) Polyclonal Antibody

Spezifität: Phospho-LEF-1 (S42) Polyclonal Antibody detects endogenous levels of LEF-1 protein only when phosphorylated at S42.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human LEF-1 Phospho-Ser42

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Andere Bezeichnung: LEF-1

Hintergrund: LEF1, Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alphaLEF1 (lymphoid enhancer binding factor 1) encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in LEF1 have been found in somatic sebaceous tumors. LEF1 has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 55kD

Gen-ID: 51176

UniProt: Q9UJU2

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding