LIM Domain Kinase 1 Antikörper

Produktnummer ABIN7222218

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LIM Domain Kinase 1 in WB, ELISA, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für LIM Domain Kinase 1 Antikörper (ABIN7222218)

Target: LIM Domain Kinase 1 (LIMK1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIM Domain Kinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-LIM Domain Kinase 1 Antikörper

Verwendungszweck: Rabbit Anti-LIMK-1 Polyclonal Antibody

Spezifität: LIMK-1 Polyclonal Antibody detects endogenous levels of LIMK-1 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human LIMK-1 around the non-phosphorylation site of T508

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Andere Bezeichnung: LIMK-1

Hintergrund: LIMK1, LIMK, LIM domain kinase 1, LIMK-1There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molekulargewicht: 65kD

Gen-ID: 3984

UniProt: P53667

Pathways: Caspase Kaskade in der Apoptose, Regulation of Cell Size, CXCR4-mediated Signaling Events