Lamin A/C Antikörper (Cleaved-Asn231)

Produktnummer ABIN7222236

Der Kaninchen Polyklonal Anti-Lamin A/C Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, Lamin A/C in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Lamin A/C Antikörper (Cleaved-Asn231) (ABIN7222236)

Target: Lamin A/C (LMNA)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Lamin A/C Antikörper

Bindungsspezifität: AA 180-260, Cleaved-Asn231

Verwendungszweck: Cleaved-Lamin A (N231) Polyclonal Antibody

Spezifität: Cleaved-Lamin A (N231) Polyclonal Antibody detects endogenous levels of fragment of activated Lamin A protein resulting from cleavage adjacent to N231.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Lamin A at AA range: 180-260

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: Lamin A

Hintergrund: Rabbit Anti-Cleaved-Lamin A (N231) Polyclonal Antibody,LMNA, LMN1, Prelamin-A/C,The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in LMNA lead to several diseases: emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.,Prelamin-A

Molekulargewicht: observerd band 50kDa

Gen-ID: 4000

UniProt: P02545

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus