ITCH Antikörper (pTyr420)

Produktnummer ABIN7225314

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ITCH in ELISA, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für ITCH Antikörper (pTyr420) (ABIN7225314)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ITCH Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-ITCH Antikörper

Bindungsspezifität: pTyr420

Verwendungszweck: Rabbit Anti-AIP4 (phospho Tyr420) Polyclonal Antibody

Spezifität: Phospho-AIP4 (Y420) Polyclonal Antibody detects endogenous levels of AIP4 protein only when phosphorylated at Y420.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human AIP4 Phospho-Tyr420

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Andere Bezeichnung: AIP4

Hintergrund: ITCH, E3 ubiquitin-protein ligase Itchy homolog, Itch, Atrophin-1-interacting protein 4, AIP4, NFE2-associated polypeptide 1, NAPP1ITCH (itchy E3 ubiquitin protein ligase) encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in ITCH are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for ITCH.

Gen-ID: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events