HMGB1 Antikörper (acLys12)

Produktnummer ABIN7212652

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch HMGB1 in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für HMGB1 Antikörper (acLys12) (ABIN7212652)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-HMGB1 Antikörper

Bindungsspezifität: acLys12

Verwendungszweck: Rabbit Anti-HMG-1 (Acetyl Lys12) Polyclonal Antibody

Spezifität: Acetyl-HMG-1 (K12) Polyclonal Antibody detects endogenous levels of HMG-1 protein only when non-acetylation at K12.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human HMG-1 around the acetylation site of K12

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:10000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: HMG-1

Hintergrund: HMGB1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1HMGB1 encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. High mobility group box 1 plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of HMGB1 have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Molekulargewicht: 30kD

Gen-ID: 3146

UniProt: P09429

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome