GSPT1 Antikörper (AA 70-150)

Produktnummer ABIN7221384

Der Kaninchen Polyklonal anti-GSPT1 Antikörper wird verwendet zum Nachweis von GSPT1 in Proben von Human, Maus und Ratte. Er wurde validiert für ELISA, IHC, WB und IF.

Kurzübersicht für GSPT1 Antikörper (AA 70-150) (ABIN7221384)

Target: GSPT1 (G1 To S Phase Transition 1 (GSPT1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GSPT1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-GSPT1 Antikörper

Bindungsspezifität: AA 70-150

Verwendungszweck: Rabbit Anti-eRF3a Polyclonal Antibody

Spezifität: ERF3a Polyclonal Antibody detects endogenous levels of eRF3a protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human eRF3a at AA range: 70-150

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu GSPT1

Target: GSPT1 (G1 To S Phase Transition 1 (GSPT1))

Andere Bezeichnung: eRF3a

Hintergrund: GSPT1, ERF3A, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homologThe eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. GSPT1 is one subunit of eRF3[PMID: 15917414, 12923185]. It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1.

Molekulargewicht: 55kD

Gen-ID: 2935

UniProt: P15170