EIF4E Antikörper (pSer209)

Produktnummer ABIN7212948

Der Kaninchen Polyklonal anti-EIF4E Antikörper wird verwendet zum Nachweis von EIF4E in Proben von Human, Ratte und Maus. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für EIF4E Antikörper (pSer209) (ABIN7212948)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4E Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-EIF4E Antikörper

Bindungsspezifität: pSer209

Verwendungszweck: Rabbit Anti-eIF4E (phospho Ser209) Polyclonal Antibody

Spezifität: Phospho-eIF4E (S209) Polyclonal Antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human eIF4E Phospho-Ser209

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Andere Bezeichnung: eIF4E

Hintergrund: EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding proteinEukaryotic translation initiation factor 4E encoded by EIF4E is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs.Eukaryotic translation initiation factor 4E aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. EIF4E acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of EIF4E are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 25kD

Gen-ID: 1977

UniProt: P06730

Pathways: BCR Signaling