EIF4B Antikörper (pSer422)

Produktnummer ABIN7221930

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch EIF4B in WB, ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für EIF4B Antikörper (pSer422) (ABIN7221930)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EIF4B Antikörper

Bindungsspezifität: pSer422

Verwendungszweck: eIF4B (phospho Ser422) Polyclonal Antibody

Spezifität: Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human eIF4B Phospho-Ser422

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Andere Bezeichnung: eIF4B

Hintergrund: Rabbit Anti-eIF4B (phospho Ser422) Polyclonal Antibody,EIF4B, Eukaryotic translation initiation factor 4B, eIF-4B,Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens , and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.,Eukaryotic translation initiation factor 4B

Molekulargewicht: observerd band 80kDa

Gen-ID: 3716

UniProt: P23588