E2F4 Antikörper (acLys96)

Produktnummer ABIN7212590

Dieser Anti-E2F4 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von E2F4 in WB und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für E2F4 Antikörper (acLys96) (ABIN7212590)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-E2F4 Antikörper

Bindungsspezifität: acLys96

Verwendungszweck: E2F-4 (Acetyl Lys96) Polyclonal Antibody

Spezifität: Acetyl-E2F-4 (K96) Polyclonal Antibody detects endogenous levels of E2F-4 protein only when acetylated at K96.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the human E2F-4 around the acetylation site of K96

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Andere Bezeichnung: E2F-4

Hintergrund: Rabbit Anti-E2F-4 (Acetyl Lys96) Polyclonal Antibody,E2F4, Transcription factor E2F4, E2F-4,The protein encoded by E2F4 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. E2F transcription factor 4 binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.,Transcription factor E2F4

Molekulargewicht: observerd band 43kDa

Gen-ID: 1874

UniProt: Q16254

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size