E2F2 Antikörper (AA 190-270)

Produktnummer ABIN7214646

Der Kaninchen Polyklonal Anti-E2F2 Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, E2F2 in Proben von Human und Maus zu detektieren.

Kurzübersicht für E2F2 Antikörper (AA 190-270) (ABIN7214646)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-E2F2 Antikörper

Bindungsspezifität: AA 190-270

Verwendungszweck: E2F-2 Polyclonal Antibody

Spezifität: E2F-2 Polyclonal Antibody detects endogenous levels of E2F-2 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human E2F-2 at AA range: 190-270

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Andere Bezeichnung: E2F-2

Hintergrund: Rabbit Anti-E2F-2 Polyclonal Antibody,E2F2, Transcription factor E2F2, E2F-2,E2F transcription factor 2 encoded by E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.,Transcription factor E2F2

Molekulargewicht: observerd band 48kDa

Gen-ID: 1870

UniProt: Q14209

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication