CRYAB Antikörper (pSer45)

Produktnummer ABIN7219702

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch CRYAB in WB, ELISA und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus und Affe.

Kurzübersicht für CRYAB Antikörper (pSer45) (ABIN7219702)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reaktivität: Human, Ratte, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CRYAB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CRYAB Antikörper

Bindungsspezifität: pSer45

Verwendungszweck: Crystallin-αB (phospho Ser45) Polyclonal Antibody

Spezifität: Phospho-Crystallin-αB (S45) Polyclonal Antibody detects endogenous levels of Crystallin-αB protein only when phosphorylated at S45.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Crystallin-alphaB Phospho-Ser45

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Andere Bezeichnung: Crystallin-alphaB

Hintergrund: Rabbit Anti-Crystallin-αB (phospho Ser45) Polyclonal Antibody,CRYAB, CRYA2, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component,Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits, the alpha-A and alpha-B subunits have a 3: 1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The crystallin alpha B has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed, alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases, a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.,Alpha-crystallin B chain

Molekulargewicht: observerd band 24kDa

Gen-ID: 1410

UniProt: P02511