Cortactin Antikörper (pTyr421)

Produktnummer ABIN7220266

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Cortactin in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für Cortactin Antikörper (pTyr421) (ABIN7220266)

Target: Cortactin (CTTN)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cortactin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-Cortactin Antikörper

Bindungsspezifität: pTyr421

Verwendungszweck: Rabbit Anti-Cortactin (phospho Tyr421) Polyclonal Antibody

Spezifität: Phospho-Cortactin (Y421) Polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Y421.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cortactin Phospho-Tyr421

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Cortactin

Target: Cortactin (CTTN)

Andere Bezeichnung: Cortactin

Hintergrund: CTTN, EMS1, Src substrate cortactin, Amplaxin, Oncogene EMS1CTTN is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The cortactin is localized in the cytoplasm and in areas of the cell-substratum contacts. CTTN has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Molekulargewicht: 80kD

Gen-ID: 2017

UniProt: Q14247

Pathways: MAPK Signalweg