Caspase 3 Antikörper (AA 20-100)

Produktnummer ABIN7212202

Der Maus Monoklonal anti-Caspase 3 Antikörper wird verwendet zum Nachweis von Caspase 3 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC (p) und IF (p).

Kurzübersicht für Caspase 3 Antikörper (AA 20-100) (ABIN7212202)

Target: Caspase 3 (CASP3)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Klon: 5H10

Produktdetails anti-Caspase 3 Antikörper

Bindungsspezifität: AA 20-100

Verwendungszweck: Mouse Anti-Caspase-3 Mouse Monoclonal Antibody (5H10)

Spezifität: Caspase-3 protein detects endogenous levels of CASP3.

Aufreinigung: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide of Caspase-3 at AA range of 20-100

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB: 500-2000,IHC 1:100-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase-3

Hintergrund: CASP3CASP3 (caspase 3) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of CASP3 results in two transcript variants that encode the same protein.

Molekulargewicht: 17, 32

Gen-ID: 836

UniProt: P42574

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation