CAD Antikörper

Produktnummer ABIN7225038

Der Kaninchen Polyklonal anti-CAD Antikörper wird verwendet zum Nachweis von CAD in Proben von Human und Maus. Er wurde validiert für IHC, ELISA und IF.

Kurzübersicht für CAD Antikörper (ABIN7225038)

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAD Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-CAD Antikörper

Verwendungszweck: Rabbit Anti-CAD Polyclonal Antibody

Spezifität: CAD Polyclonal Antibody detects endogenous levels of CAD protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CAD around the non-phosphorylation site of T456

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CAD

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Andere Bezeichnung: CAD

Hintergrund: CAD, CAD proteinThe de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 250kD

Gen-ID: 790

UniProt: P27708

Pathways: Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process